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Pi Binding

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Pi Binding. Pi binds to dna by intercalating between the bases with little or no sequence preference. Two p orbitals forming a π bond.

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Pi bonds can form in double and triple bonds but do not form in single bonds in most cases. Pi bonds are often written as 𝛑 bonds where the greek letter 𝛑 refers to the similar symmetry of the pi bond and the p orbital. Each of these atomic orbitals has zero electron density at a shared nodal plane passing through the two bonded nuclei.

The same plane is also a nodal plane for the molecular orbital of the pi bond.

Pi binds to dna by intercalating between the bases with little or no sequence preference. After binding dna the quantum yield of pi is enhanced 20 30 fold and the excitation emission maximum of pi is shifted to 535 nm green 617 nm orange red. A pair of atoms may be connected by one or by two pi bonds only if a sigma bond also exists between them. Pi bond in chemistry a cohesive interaction between two atoms and a pair of electrons that occupy an orbital located in two regions roughly parallel to the line determined by the two atoms.

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